Construction and Detection of Recombinant Adeno-Associated Virus Co- Expressing PRRSV GP5, M Proteins and shRNA.

Porcine reproductive and respiratory syndrome (PRRS) is a contagious disease of swine caused by porcine reproductive and respiratory syndrome virus (PRRSV). PRRS wasfirst reported in America in the late 1980s and subsequently reported in Europe. The main clinical symptoms of this highly pathogenic disease are abortion, stillbirth, breeding problems, and respiratory problems in newborns. The vaccines that are currently commercially available contain inactivated or attenuated virus immunogens and can lessen the clinical severity of PRRS. 

However, these regimens offer limited protective efficacy and have a poor safety profile. Design of a more effective immunogen or regimen is limited by the lack of information available about the immune response to PRRSV. The protective immune mechanism elicited by current vaccines is undetermined, and the immune mechanisms responsible for clearing a natural infection of PRRSV remain unknown. Furthermore, an efficacious immunogen must elicit an immune response capable of protecting animals from genetically diverse strains of this virus.(Read more)